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Mouse Anti B Actin Hrp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti mouse b actin  (Santa Cruz Biotechnology)
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a. qPCR analysis of Mif expression in VO-PyMT cells transduced with control or two independent Mif-targeting short hairpin RNAs (shRNA). Bar heights show means and error bars depict standard deviation of technical triplicates. Data was reproduced in 3 independent experiments. b. Immunoblot of Mif protein in shControl- or shMif-transduced VO-PyMT <t>cells.</t> <t>B-Actin</t> is shown as loading control. Data is representative of two independent experiments. c. MIF protein concentration in cell culture supernatants of shControl and shMif-transduced VO-PyMT cells as determined by enzyme linked immunosorbent assay (ELISA). Bar heights depict means and error bars show standard deviation of two technical replicates. d. Relative Mif expression levels in Py8119 cells harboring an shControl or shMif hairpin, as determined by qPCR. Error bars show standard deviations. Data is representative of 3 separate experiments. e. Western blot analysis of MIF protein in shControl or shMif Py8119 cells. B-Actin is shown as loading control. Data is representative of two independent experiments. f. Number of spheres per well quantified in in vitro spheroid cultures of VO-PyMT or Py8119 cells upon Mif knockdown. Sphere count was performed 7 days post seeding. Ns, not significant. Two-tailed Student’s t tests were used to estimate significance. g. Representative images of spheroids quantified in (f). Scale bars: 1 mm.
Mouse Anti Mouse B Actin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a. qPCR analysis of Mif expression in VO-PyMT cells transduced with control or two independent Mif-targeting short hairpin RNAs (shRNA). Bar heights show means and error bars depict standard deviation of technical triplicates. Data was reproduced in 3 independent experiments. b. Immunoblot of Mif protein in shControl- or shMif-transduced VO-PyMT <t>cells.</t> <t>B-Actin</t> is shown as loading control. Data is representative of two independent experiments. c. MIF protein concentration in cell culture supernatants of shControl and shMif-transduced VO-PyMT cells as determined by enzyme linked immunosorbent assay (ELISA). Bar heights depict means and error bars show standard deviation of two technical replicates. d. Relative Mif expression levels in Py8119 cells harboring an shControl or shMif hairpin, as determined by qPCR. Error bars show standard deviations. Data is representative of 3 separate experiments. e. Western blot analysis of MIF protein in shControl or shMif Py8119 cells. B-Actin is shown as loading control. Data is representative of two independent experiments. f. Number of spheres per well quantified in in vitro spheroid cultures of VO-PyMT or Py8119 cells upon Mif knockdown. Sphere count was performed 7 days post seeding. Ns, not significant. Two-tailed Student’s t tests were used to estimate significance. g. Representative images of spheroids quantified in (f). Scale bars: 1 mm.
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mouse anti b actin  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology mouse anti b actin
a. qPCR analysis of Mif expression in VO-PyMT cells transduced with control or two independent Mif-targeting short hairpin RNAs (shRNA). Bar heights show means and error bars depict standard deviation of technical triplicates. Data was reproduced in 3 independent experiments. b. Immunoblot of Mif protein in shControl- or shMif-transduced VO-PyMT <t>cells.</t> <t>B-Actin</t> is shown as loading control. Data is representative of two independent experiments. c. MIF protein concentration in cell culture supernatants of shControl and shMif-transduced VO-PyMT cells as determined by enzyme linked immunosorbent assay (ELISA). Bar heights depict means and error bars show standard deviation of two technical replicates. d. Relative Mif expression levels in Py8119 cells harboring an shControl or shMif hairpin, as determined by qPCR. Error bars show standard deviations. Data is representative of 3 separate experiments. e. Western blot analysis of MIF protein in shControl or shMif Py8119 cells. B-Actin is shown as loading control. Data is representative of two independent experiments. f. Number of spheres per well quantified in in vitro spheroid cultures of VO-PyMT or Py8119 cells upon Mif knockdown. Sphere count was performed 7 days post seeding. Ns, not significant. Two-tailed Student’s t tests were used to estimate significance. g. Representative images of spheroids quantified in (f). Scale bars: 1 mm.
Mouse Anti B Actin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a. qPCR analysis of Mif expression in VO-PyMT cells transduced with control or two independent Mif-targeting short hairpin RNAs (shRNA). Bar heights show means and error bars depict standard deviation of technical triplicates. Data was reproduced in 3 independent experiments. b. Immunoblot of Mif protein in shControl- or shMif-transduced VO-PyMT <t>cells.</t> <t>B-Actin</t> is shown as loading control. Data is representative of two independent experiments. c. MIF protein concentration in cell culture supernatants of shControl and shMif-transduced VO-PyMT cells as determined by enzyme linked immunosorbent assay (ELISA). Bar heights depict means and error bars show standard deviation of two technical replicates. d. Relative Mif expression levels in Py8119 cells harboring an shControl or shMif hairpin, as determined by qPCR. Error bars show standard deviations. Data is representative of 3 separate experiments. e. Western blot analysis of MIF protein in shControl or shMif Py8119 cells. B-Actin is shown as loading control. Data is representative of two independent experiments. f. Number of spheres per well quantified in in vitro spheroid cultures of VO-PyMT or Py8119 cells upon Mif knockdown. Sphere count was performed 7 days post seeding. Ns, not significant. Two-tailed Student’s t tests were used to estimate significance. g. Representative images of spheroids quantified in (f). Scale bars: 1 mm.
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a. qPCR analysis of Mif expression in VO-PyMT cells transduced with control or two independent Mif-targeting short hairpin RNAs (shRNA). Bar heights show means and error bars depict standard deviation of technical triplicates. Data was reproduced in 3 independent experiments. b. Immunoblot of Mif protein in shControl- or shMif-transduced VO-PyMT <t>cells.</t> <t>B-Actin</t> is shown as loading control. Data is representative of two independent experiments. c. MIF protein concentration in cell culture supernatants of shControl and shMif-transduced VO-PyMT cells as determined by enzyme linked immunosorbent assay (ELISA). Bar heights depict means and error bars show standard deviation of two technical replicates. d. Relative Mif expression levels in Py8119 cells harboring an shControl or shMif hairpin, as determined by qPCR. Error bars show standard deviations. Data is representative of 3 separate experiments. e. Western blot analysis of MIF protein in shControl or shMif Py8119 cells. B-Actin is shown as loading control. Data is representative of two independent experiments. f. Number of spheres per well quantified in in vitro spheroid cultures of VO-PyMT or Py8119 cells upon Mif knockdown. Sphere count was performed 7 days post seeding. Ns, not significant. Two-tailed Student’s t tests were used to estimate significance. g. Representative images of spheroids quantified in (f). Scale bars: 1 mm.
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a. qPCR analysis of Mif expression in VO-PyMT cells transduced with control or two independent Mif-targeting short hairpin RNAs (shRNA). Bar heights show means and error bars depict standard deviation of technical triplicates. Data was reproduced in 3 independent experiments. b. Immunoblot of Mif protein in shControl- or shMif-transduced VO-PyMT <t>cells.</t> <t>B-Actin</t> is shown as loading control. Data is representative of two independent experiments. c. MIF protein concentration in cell culture supernatants of shControl and shMif-transduced VO-PyMT cells as determined by enzyme linked immunosorbent assay (ELISA). Bar heights depict means and error bars show standard deviation of two technical replicates. d. Relative Mif expression levels in Py8119 cells harboring an shControl or shMif hairpin, as determined by qPCR. Error bars show standard deviations. Data is representative of 3 separate experiments. e. Western blot analysis of MIF protein in shControl or shMif Py8119 cells. B-Actin is shown as loading control. Data is representative of two independent experiments. f. Number of spheres per well quantified in in vitro spheroid cultures of VO-PyMT or Py8119 cells upon Mif knockdown. Sphere count was performed 7 days post seeding. Ns, not significant. Two-tailed Student’s t tests were used to estimate significance. g. Representative images of spheroids quantified in (f). Scale bars: 1 mm.
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mouse monoclonal anti b actin  (Cell Signaling Technology Inc)
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a. qPCR analysis of Mif expression in VO-PyMT cells transduced with control or two independent Mif-targeting short hairpin RNAs (shRNA). Bar heights show means and error bars depict standard deviation of technical triplicates. Data was reproduced in 3 independent experiments. b. Immunoblot of Mif protein in shControl- or shMif-transduced VO-PyMT <t>cells.</t> <t>B-Actin</t> is shown as loading control. Data is representative of two independent experiments. c. MIF protein concentration in cell culture supernatants of shControl and shMif-transduced VO-PyMT cells as determined by enzyme linked immunosorbent assay (ELISA). Bar heights depict means and error bars show standard deviation of two technical replicates. d. Relative Mif expression levels in Py8119 cells harboring an shControl or shMif hairpin, as determined by qPCR. Error bars show standard deviations. Data is representative of 3 separate experiments. e. Western blot analysis of MIF protein in shControl or shMif Py8119 cells. B-Actin is shown as loading control. Data is representative of two independent experiments. f. Number of spheres per well quantified in in vitro spheroid cultures of VO-PyMT or Py8119 cells upon Mif knockdown. Sphere count was performed 7 days post seeding. Ns, not significant. Two-tailed Student’s t tests were used to estimate significance. g. Representative images of spheroids quantified in (f). Scale bars: 1 mm.
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mouse anti beta actin  (Proteintech)
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a. qPCR analysis of Mif expression in VO-PyMT cells transduced with control or two independent Mif-targeting short hairpin RNAs (shRNA). Bar heights show means and error bars depict standard deviation of technical triplicates. Data was reproduced in 3 independent experiments. b. Immunoblot of Mif protein in shControl- or shMif-transduced VO-PyMT <t>cells.</t> <t>B-Actin</t> is shown as loading control. Data is representative of two independent experiments. c. MIF protein concentration in cell culture supernatants of shControl and shMif-transduced VO-PyMT cells as determined by enzyme linked immunosorbent assay (ELISA). Bar heights depict means and error bars show standard deviation of two technical replicates. d. Relative Mif expression levels in Py8119 cells harboring an shControl or shMif hairpin, as determined by qPCR. Error bars show standard deviations. Data is representative of 3 separate experiments. e. Western blot analysis of MIF protein in shControl or shMif Py8119 cells. B-Actin is shown as loading control. Data is representative of two independent experiments. f. Number of spheres per well quantified in in vitro spheroid cultures of VO-PyMT or Py8119 cells upon Mif knockdown. Sphere count was performed 7 days post seeding. Ns, not significant. Two-tailed Student’s t tests were used to estimate significance. g. Representative images of spheroids quantified in (f). Scale bars: 1 mm.
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a. qPCR analysis of Mif expression in VO-PyMT cells transduced with control or two independent Mif-targeting short hairpin RNAs (shRNA). Bar heights show means and error bars depict standard deviation of technical triplicates. Data was reproduced in 3 independent experiments. b. Immunoblot of Mif protein in shControl- or shMif-transduced VO-PyMT cells. B-Actin is shown as loading control. Data is representative of two independent experiments. c. MIF protein concentration in cell culture supernatants of shControl and shMif-transduced VO-PyMT cells as determined by enzyme linked immunosorbent assay (ELISA). Bar heights depict means and error bars show standard deviation of two technical replicates. d. Relative Mif expression levels in Py8119 cells harboring an shControl or shMif hairpin, as determined by qPCR. Error bars show standard deviations. Data is representative of 3 separate experiments. e. Western blot analysis of MIF protein in shControl or shMif Py8119 cells. B-Actin is shown as loading control. Data is representative of two independent experiments. f. Number of spheres per well quantified in in vitro spheroid cultures of VO-PyMT or Py8119 cells upon Mif knockdown. Sphere count was performed 7 days post seeding. Ns, not significant. Two-tailed Student’s t tests were used to estimate significance. g. Representative images of spheroids quantified in (f). Scale bars: 1 mm.

Journal: bioRxiv

Article Title: Single-cell profiling of synchronous multi-organ metastasis reveals a systemic CD74 + lipid-associated macrophage niche driving polymetastatic breast cancer

doi: 10.64898/2026.01.31.701004

Figure Lengend Snippet: a. qPCR analysis of Mif expression in VO-PyMT cells transduced with control or two independent Mif-targeting short hairpin RNAs (shRNA). Bar heights show means and error bars depict standard deviation of technical triplicates. Data was reproduced in 3 independent experiments. b. Immunoblot of Mif protein in shControl- or shMif-transduced VO-PyMT cells. B-Actin is shown as loading control. Data is representative of two independent experiments. c. MIF protein concentration in cell culture supernatants of shControl and shMif-transduced VO-PyMT cells as determined by enzyme linked immunosorbent assay (ELISA). Bar heights depict means and error bars show standard deviation of two technical replicates. d. Relative Mif expression levels in Py8119 cells harboring an shControl or shMif hairpin, as determined by qPCR. Error bars show standard deviations. Data is representative of 3 separate experiments. e. Western blot analysis of MIF protein in shControl or shMif Py8119 cells. B-Actin is shown as loading control. Data is representative of two independent experiments. f. Number of spheres per well quantified in in vitro spheroid cultures of VO-PyMT or Py8119 cells upon Mif knockdown. Sphere count was performed 7 days post seeding. Ns, not significant. Two-tailed Student’s t tests were used to estimate significance. g. Representative images of spheroids quantified in (f). Scale bars: 1 mm.

Article Snippet: Primary antibodies included: rabbit anti-mouse Mif (1:1000, Abcam, ab187064) and mouse anti-mouse B-actin (1:1000, Santa Cruz, sc-47778).

Techniques: Expressing, Transduction, Control, shRNA, Standard Deviation, Western Blot, Protein Concentration, Cell Culture, Enzyme-linked Immunosorbent Assay, In Vitro, Knockdown, Two Tailed Test